Title Single-channel Array Normalization (scan) and University Probability of Expression Codes (upc)
نویسندگان
چکیده
Description SCAN is a microarray normalization method to facilitate personalized-medicine work-flows. Rather than processing microarray samples as groups, which can introduce biases and present logistical challenges, SCAN normalizes each sample individually by model-ing and removing probe-and array-specific background noise using only data from within each array. SCAN can be applied to one-channel (e.g., Affymetrix) or two-channel (e.g., Agilent) mi-croarrays. The Universal Probability of expression Codes (UPC) method is an extension of SCAN that generates probability-of-expression values. These values can be interpreted as the probability that a given genomic feature (e.g., gene, transcript) is expressed above the background in a given sample. The UPC method can be applied to one-channel or two-channel microarrays as well as to RNA-Seq read counts. Because UPC values are represented on the same scale and have an identical interpretation for each platform , they can be used for cross-platform data integration.) License MIT
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